Increase in copy number of an integrated vector during continuous culture of Hansenula polymorpha expressing functional human haemoglobin.

@article{Gilbert1994IncreaseIC,
  title={Increase in copy number of an integrated vector during continuous culture of Hansenula polymorpha expressing functional human haemoglobin.},
  author={Sabine Gilbert and Hero van Urk and Andrew J. Greenfield and Michael J McAvoy and K A Denton and Dr. B. A. Coghlan and George D. D. Jones and David J. Mead},
  journal={Yeast},
  year={1994},
  volume={10 12},
  pages={1569-80}
}
Recombinant human haemoglobin A (rHbA) was produced by a leucine-requiring strain of Hansenula polymorpha which had been transformed with an integration vector containing the Saccharomyces cerevisiae LEU2 gene and cDNAs for the expression of alpha and beta globin each driven by the H. polymorpha MOX promoter. After 40 generations in a chemostat it was found that the integrated vector had become amplified in the host strain. In some cases this led to an increase in LEU2 gene dosage, but a loss… CONTINUE READING