Increase in ascorbate content of transgenic tobacco plants overexpressing the acerola (Malpighia glabra) phosphomannomutase gene.

@article{Badejo2009IncreaseIA,
  title={Increase in ascorbate content of transgenic tobacco plants overexpressing the acerola (Malpighia glabra) phosphomannomutase gene.},
  author={Adebanjo Ayobamidele Badejo and Hani Ahmed Eltelib and Kazunari Fukunaga and Yukichi Fujikawa and Muneharu Esaka},
  journal={Plant \& cell physiology},
  year={2009},
  volume={50 2},
  pages={
          423-8
        }
}
Phosphomannomutase (PMM; EC 5.4.2.8) catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate in the Smirnoff-Wheeler pathway for the biosynthesis of l-ascorbic acid (AsA). We have cloned the PMM cDNA from acerola (Malpighia glabra), a plant containing an enormous amount of AsA. The AsA contents correlate with the PMM gene expression of the ripening fruits and leaves. The PMM activities in the leaves of acerola, tomato and Arabidopsis correlate with their respective AsA… 
Overexpression of the acerola (Malpighia glabra) monodehydroascorbate reductase gene in transgenic tobacco plants results in increased ascorbate levels and enhanced tolerance to salt stress
TLDR
Results indicate that overexpression of acerola MDHAR imparts greater tolerance to salt stress and is a key enzyme of the ascorbate (AsA)-glutathione cycle that maintains reduced pools of AsA and serves as an important antioxidative enzyme.
Overexpression of the Monodehydroascorbate Reductase Gene from Non-heading Chinese Cabbage Reduces Ascorbate Level and Growth in Transgenic Tobacco
TLDR
Cl cloning of the reductase gene from non-heading Chinese cabbage and negative regulation of l-ascorbic acid and dehydroascorbate levels in transgenic tobacco plants expressing the gene under the control of the 35S promoter are reported.
Full Length Research Article ENHANCED SALT-MEDIATED OXIDATIVE STRESS TOLERANCE IN TRANSGENIC TOBACCO OVEREXPRESSING ACEROLA GDP-L-GALACTOSE PHOSPHORYLASE AND PHOSPHOMANNOMUTASE GENES
TLDR
Results indicate that overexpressing of acerola GGP and PMM confers greater protection for transgenic plants under oxidative stress conditions mediated by salt.
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TLDR
This chapter focuses on expression and regulation of genes involved in AsA biosynthesis of higher plants and proposes that high transcriptional expression level of the biosynthetic enzymes may contribute to high accumulation of AsA.
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TLDR
Results suggest that this modulation point of the ascorbate biosynthesis could be potentially used for biotechnological purposes to increase the vitamin C levels in pepper fruits.
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TLDR
Results suggest that GGP/VTC2 acts as a rate-limiting step regulating AsA biosynthesis in response to light and dark conditions.
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The fruit of Actinidia are unusual in that they contain very high concentrations of vitamin C—over 80 mg/100 g fresh weight (FW) in commercial cultivars and over 800 mg/100 g FW in some wild species.
Molecular cloning and functional analysis of the phosphomannomutase (PMM) gene from Dendrobium officinale and evidence for the involvement of an abiotic stress response during germination
TLDR
Genetic evidence for the involvement of PMM genes in the biosynthesis of AsA and polysaccharides and the mediation of PMm genes in abiotic stress tolerance during seed germination in A. thaliana is provided.
The Role of Plant High-Throughput Phenotyping in the Characterization of the Response of High Ascorbate Plants to Abiotic Stresses
TLDR
An overview of how the use of high-throughput phenotyping or phenomics is revolutionizing the way plant phenotypes are characterized is presented and the power of digital phenotypes in the characterization of plants overexpressing enzymes in the inositol pathway to ascorbate is illustrated.
Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola (Malpighia glabra).
TLDR
MDHAR and DHAR transcripts and enzyme activities were significantly up-regulated in the leaves of acerola under cold and salt stress conditions, indicating that expression of both genes are transcriptionally regulated under these stresses.
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References

SHOWING 1-10 OF 27 REFERENCES
Gene expression of ascorbic acid biosynthesis related enzymes of the Smirnoff-Wheeler pathway in acerola (Malpighia glabra).
TLDR
Cloned some of the genes involved in the Smirnoff-Wheeler pathway from acerola, a plant containing enormous amount of AsA, and examined the expression patterns of these genes in the plant, finding correlation in their expression with the AsA contents of the fruits.
Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra) and increase in ascorbate content of transgenic tobacco expressing the acerola gene.
TLDR
It is found that the GMP mRNA levels in acerola are higher than those from Arabidopsis and tomato, and the transient expression of the uidA reporter gene in the protoplasts of Nicotiana tabacum cultures showed the MgGMP gene promoter to have higher activity than the cauliflower mosaic virus 35S andArabidopsis GMP promoters.
Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana.
TLDR
Improved understanding on the molecular and functional properties of plant PMM and genetic evidence on the involvement of PMM in the biosynthesis of AsA in Arabidopsis and N. benthamiana plants are provided.
A Temperature-sensitive Mutation in the Arabidopsis thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death*
TLDR
It is proposed that a deficiency in protein glycosylation is responsible for the observed cell death phenotype of the temperature-sensitive Arabidopsis thaliana pmm-12 mutant.
Cloning and expression of GDP-D-mannose pyrophosphorylase gene and ascorbic acid content of acerola (Malpighia glabra L.) fruit at ripening stages.
Acerola (Malpighia glabra L.) is one of the richest natural sources of L-ascorbic acid (AsA; vitamin C). GDP-D-mannose pyrophosphorylase (GMP; EC 2.7.7.13) was found to play a major role in the
Ascorbate content of wheat leaves is not determined by maximal l‐galactono‐1,4‐lactone dehydrogenase (GalLDH) activity under drought stress
TLDR
The results suggest that GalLDH protein and activity cannot be used as an indicator for changes in the capacity for ascorbate biosynthesis and that AA biosynthesis is constrained by other factors under stress.
Synthesis of L-ascorbic acid in the phloem
TLDR
The novel finding that active AsA biosynthesis occurs in the phloem is presented and must now be considered in the context of mechanisms implicated in whole plant AsA distribution.
L-Ascorbic acid accumulation in fruit of Ribes nigrum occurs by in situ biosynthesis via the L-galactose pathway.
TLDR
Data suggest strong developmental, metabolic and genetic control of AsA accumulation in blackcurrant fruit and indicate the potential for breeding high AsA cultivars.
Arabidopsis Phosphomannose Isomerase 1, but Not Phosphomannose Isomerase 2, Is Essential for Ascorbic Acid Biosynthesis*
TLDR
Improved understanding of the molecular and functional properties of Arabidopsis PMI isoenzymes are improved and genetic evidence of the involvement of PMI1, but not PMI2, in the biosynthesis of AsA inArabidopsis plants is provided.
Identification of ascorbic acid-deficient Arabidopsis thaliana mutants.
TLDR
A novel AsA assay is used to isolate four vtc mutants that define three additional VTC loci, and a second mutant allele of VTC1 is isolated, providing strong evidence for the recently proposed role of GDP-mannose in AsA biosynthesis.
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