Incorporation of unnatural non-α-amino acids into the N-terminus of proteins in a cell-free translation system.

Abstract

Unnatural amino acid mutagenesis allows us to introduce unnatural α-amino acids into internal positions of proteins in response to expanded codons such as amber and four-base codons. To improve the unnatural amino acid mutagenesis, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus of proteins in a cell-free translation system.

DOI: 10.1007/978-1-61779-331-8_14

Cite this paper

@article{Hohsaka2012IncorporationOU, title={Incorporation of unnatural non-α-amino acids into the N-terminus of proteins in a cell-free translation system.}, author={Takahiro Hohsaka}, journal={Methods in molecular biology}, year={2012}, volume={794}, pages={229-39} }