Incorporation of the Whole Chromosomal DNA in Protoplast Lysates into Competent Cells of Bacillus subtilis

@article{Akamatsu2001IncorporationOT,
  title={Incorporation of the Whole Chromosomal DNA in Protoplast Lysates into Competent Cells of Bacillus subtilis},
  author={T. Akamatsu and H. Taguchi},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2001},
  volume={65},
  pages={823 - 829}
}
Competent cells of Bacillus subtilis AC870 (purB, leuB, trpC, ald-1) were transformed to Ade+, Trp+, or Ade+ Trp+ with DNA in protoplast lysates of B. subtilis AC819 (hisH, tet-1, rpsL, smo-1). The contransfer ratio of purB to trpC was constant at 7-9% (Ade+ Trp+/Trp+) or 3% (Ade+ Trp+/Ade+) at protoplast concentrations of 2.7×103∼2.7×106 per ml. The whole chromosomal DNA must be certainly incorporated into competent cells from the following reasons; (1) purB is opposite to trpC on the… Expand
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