Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability.

@article{Karik2008IncorporationOP,
  title={Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability.},
  author={Katalin Karik{\'o} and Hisafumi Muramatsu and Frank A. Welsh and J{\'a}nos Ludwig and Hiroki Kato and Shizuo Akira and Drew Weissman},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2008},
  volume={16 11},
  pages={
          1833-40
        }
}
In vitro-transcribed mRNAs encoding physiologically important proteins have considerable potential for therapeutic applications. However, in its present form, mRNA is unfeasible for clinical use because of its labile and immunogenic nature. Here, we investigated whether incorporation of naturally modified nucleotides into transcripts would confer enhanced biological properties to mRNA. We found that mRNAs containing pseudouridines have a higher translational capacity than unmodified mRNAs when… CONTINUE READING
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