Incorporation of proteins into (Xenopus) oocytes by proteoliposome microinjection: functional characterization of a novel aquaporin.

@article{Cahrec1996IncorporationOP,
  title={Incorporation of proteins into (Xenopus) oocytes by proteoliposome microinjection: functional characterization of a novel aquaporin.},
  author={Françoise Le Cah{\'e}rec and Patrick Bron and Jean Marc Verbavatz and Andrea Garret and Ga{\"e}lle Morel and Annie Cavalier and Georgette Bonnec and David Thomas and Jean Gouranton and John F. Hubert},
  journal={Journal of cell science},
  year={1996},
  volume={109 ( Pt 6)},
  pages={
          1285-95
        }
}
Xenopus laevis oocytes are widely used as an expression system for plasma membrane proteins, achieved by cytoplasmic microinjection of messenger RNA. In the present study, we propose an alternative system allowing functional insertion of exogenous proteins into the plasma membrane of Xenopus oocytes. We microinjected proteoliposome suspensions into the cytoplasm and then analyzed membrane protein function. The proteins used in this work were members of the MIP family: the human erythrocyte… CONTINUE READING

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