Incorporation of partial polyhedrin homology sequences (PPHS) enhances the production of cloned foreign genes in a baculovirus expression system.

@article{Gong2006IncorporationOP,
  title={Incorporation of partial polyhedrin homology sequences (PPHS) enhances the production of cloned foreign genes in a baculovirus expression system.},
  author={Zhaohui Gong and Yongfeng Jin and Yao-Zhou Zhang},
  journal={Biotechnology and applied biochemistry},
  year={2006},
  volume={43 Pt 3},
  pages={165-70}
}
Baculovirus expression vector systems (BEVSs) have been used extensively for high-level expression of cloned foreign genes. In many instances, the levels of recombinant protein(s) produced in insect cells and larvae are insufficient for experimental purposes. Thus new techniques and methods are needed to increase significantly the protein expression levels in BEVS. In the present paper, we describe the incorporation of a 15 bp element derived from the 5'-end partial sequence of the polyhedrin… CONTINUE READING