Incorporation of large quantities of phospholipid by net de novo synthesis in cytoplasmic membrane vesicles of Escherichia coli.

Abstract

De novo phospholipid synthesis has been studied in cytoplasmic membrane vesicles of Escherichia coli in the presence of sn-glycerol 3-phosphate, CTP, serine and acyl-CoA. Continuous addition of acyl-CoA was found to result in phospholipid biosynthesis during more than 4 h of incubation and phospholipid corresponding to 3--4-times the total amount of lipid… (More)

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