Incorporation of dengue virus replicon into virus-like particles by a cell line stably expressing precursor membrane and envelope proteins of dengue virus type 2.

@article{Lai2008IncorporationOD,
  title={Incorporation of dengue virus replicon into virus-like particles by a cell line stably expressing precursor membrane and envelope proteins of dengue virus type 2.},
  author={Chih-yun Lai and Hsien-Ping Hu and Chwan-Chuen King and Wei-Kung Wang},
  journal={Journal of biomedical science},
  year={2008},
  volume={15 1},
  pages={
          15-27
        }
}
While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causal agent of the most important arboviral diseases in this century. In this study, we successfully established a cell line stably expressing the precursor membrane/envelope (PrM/E… 
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References

SHOWING 1-10 OF 56 REFERENCES
Incorporation of Tick-Borne Encephalitis Virus Replicons into Virus-Like Particles by a Packaging Cell Line
TLDR
RNA replicons derived from flavivirus genomes show considerable potential as gene transfer and immunization vectors and can be used both as a source for mature TBE virus RSPs and as a safe and convenient replicon packaging cell line, providing the TBEirus surface proteins prM/M and E in trans.
trans-Packaged West Nile Virus-Like Particles: Infectious Properties In Vitro and in Infected Mosquito Vectors
TLDR
It is demonstrated that VLPs can serve as a valuable tool for the investigation of tissue tropism during the early stages of infection, where virus spread and the need for biosafety level 3 containment complicate the use of wild-type virus.
Development of Dengue virus type 2 replicons capable of prolonged expression in host cells
TLDR
D Dengue virus genomes lacking major structural proteins can, like other flaviviruses, replicate intracellularly and express virus non-structural proteins with minimal toxicity to host cells and pave the way for the development of dengue virus replicons as a form of live, attenuated virus vaccine.
Packaging the replicon RNA of the Far-Eastern subtype of tick-borne encephalitis virus into single-round infectious particles: development of a heterologous gene delivery system.
Chimeric Dengue Type 2 (Vaccine Strain PDK-53)/Dengue Type 1 Virus as a Potential Candidate Dengue Type 1 Virus Vaccine
TLDR
This study indicated that the infectious clones derived from the candidate DEN-2 PDK-53 vaccine are promising attenuated vectors for development of chimeric flavivirus vaccines.
Infectious RNA transcribed from stably cloned full-length cDNA of dengue type 4 virus.
TLDR
Evidence is presented that dengue virus recovered from permissive cells transfected with the in vitro RNA transcripts retained a mutation that was engineered into full-length cDNA that should facilitate the development of a safe and effective live vaccine for use in humans.
Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2
TLDR
The r DEN2Δ30 and rDEN2΢30-4995 viruses can be considered for evaluation in humans and for inclusion in a tetravalent dengue vaccine.
Kunjin Virus Replicon Vectors for Human Immunodeficiency Virus Vaccine Development
TLDR
KUN replicon VLP vaccinations induced long-lasting immune responses with CD8+ T cells able to secrete gamma interferon and to mediate protection 6 to 10 months after immunization, illustrating the potential value of the K UN replicon vectors for human immunodeficiency virus vaccine design.
Construction and applications of yellow fever virus replicons.
Noncytopathic flavivirus replicon RNA-based system for expression and delivery of heterologous genes.
TLDR
Noncytopathic KUN replicon vectors with the ability to be packaged into VLPs should provide a useful tool for the development of noninfectious and noncy topathic vaccines as well as for gene therapy applications.
...
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