Incorporation of adenovirus in calcium phosphate precipitates enhances gene transfer to airway epithelia in vitro and in vivo.

@article{Fasbender1998IncorporationOA,
  title={Incorporation of adenovirus in calcium phosphate precipitates enhances gene transfer to airway epithelia in vitro and in vivo.},
  author={Andreas Fasbender and Jae Ho Lee and Robert W. Walters and Thomas O. Moninger and Joseph Zabner and M. J. Welsh},
  journal={The Journal of clinical investigation},
  year={1998},
  volume={102 1},
  pages={184-93}
}
Adenovirus (Ad)-mediated gene transfer to airway epithelia is inefficient because the apical membrane lacks the receptor activity to bind adenovirus fiber protein. Calcium phosphate (CaPi) precipitates have been used to deliver plasmid DNA to cultured cell lines. However, such precipitates are not effective in many primary cultures or in vivo. Here we show that incorporating recombinant adenovirus into a CaPi coprecipitate markedly enhances transgene expression in cells that are resistant to… CONTINUE READING