Incorporation of Multiple Sequential Pseudothymidines by DNA Polymerases and Their Impact on DNA Duplex Structure

@article{Havemann2008IncorporationOM,
  title={Incorporation of Multiple Sequential Pseudothymidines by DNA Polymerases and Their Impact on DNA Duplex Structure},
  author={S. A. Havemann and S. Hoshika and Daniel Hutter and S. Benner},
  journal={Nucleosides, Nucleotides \& Nucleic Acids},
  year={2008},
  volume={27},
  pages={261 - 278}
}
Thermal denaturation and circular dichroism studies suggested that multiple (up to 12), sequential pseudothymidines, a representative C-glycoside, do not perturb the structure of a representative DNA duplex. Further, various Family A and B DNA polymerases were found to extend a primer by incorporating four sequential pseudothymidine triphosphates, and then continue the extension to generate full-length product. Detailed studies showed that Taq polymerase incorporated up to five sequential C… Expand
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