Incorporation of [3H]glucosamine into keratin-related polypeptides in pig epidermis.

@article{King1986IncorporationO,
  title={Incorporation of [3H]glucosamine into keratin-related polypeptides in pig epidermis.},
  author={I A King},
  journal={FEBS letters},
  year={1986},
  volume={201 1},
  pages={114-8}
}
Metabolic labelling studies have provided evidence for glycosylated keratins in cultured pig epidermis. [3H]Glucosamine was incorporated into five major particulate polypeptides of Mr 68 000, 61 000, 57 000, 53,000 and 48,000. Radioactivity was present in protein-bound carbohydrate. Non-enzymic glycation was excluded. Labelling was largely unaffected by tunicamycin indicating that radioactivity was incorporated mainly into O-linked oligosaccharides. These [3H]glucosamine-labelled components… CONTINUE READING

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These [ 3H]glucosamine - labelled components were closely related to keratins since they had a similar electrophoretic mobility to polypeptides of purified pig prekeratin , they were immunoprecipitated by anti - prekeratin serum and they were incorporated into reconstituted , intermediate - sized , keratin filaments .
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