Incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids in phospholipid classes in cultured rainbow trout (Salmo gairdneri) cells

@article{Tocher1990IncorporationAM,
  title={Incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids in phospholipid classes in cultured rainbow trout (Salmo gairdneri) cells},
  author={Douglas R Tocher},
  journal={Fish Physiology and Biochemistry},
  year={1990},
  volume={8},
  pages={239-249}
}
The incorporation and metabolism of various (n-3) and (n-6) polyunsaturated fatty acids supplemented to the culture medium was investigated in the rainbow trout cell line, RTG-2. The distribution, and the occurrence and relative extent of further desaturation and elongation of the incorporated acids was determined in individual phospholipid classes by analysis of the fatty acid compositions. RTG-2 cells exhibited Δ6 and Δ5 desaturase activities whereas Δ4 desaturase activity was almost totally… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 12 CITATIONS

References

Publications referenced by this paper.
SHOWING 1-10 OF 22 REFERENCES

An improved method for the addition of long - chain free fatty acids to protein solutions

D. R. Tocher, J. Carr, J. R. Sargent
  • Anal . Biochem .
  • 1989

Fatty acid composition of phospholipids and neutral lipids during embryonic and early larval development in Atlantic herring ( Clupea harengus , L . )

D. R. Tocher, D. G. Harvie
  • Lipids
  • 1988

The influence of thermal acclimation on membrane lipid composition of rainbow trout liver

R. J. Henderson, D. R. Tocher
  • 1987

Lipid metabolism

J. L. Harwood F. D. Gunstone, F. B. Padley
  • Biochim . Biophys . Acta
  • 1986

Fish lipids , part 1

M. V. Bell, R. J. Henderson, J. R. Sargent
  • 1985