Incidence of Fas positivity and deoxyribonucleic acid double-stranded breaks in human ejaculated sperm.

Abstract

OBJECTIVE To determine the incidence of Fas positivity and DNA double-strand breaks (DSB) as indicators of early- and late-stage apoptosis in ejaculated sperm. DESIGN Fas positivity was assessed by flow cytometry and DSB by the neutral Comet assay. SETTING Andrology Laboratory, Royal Maternity Hospital, Belfast, Northern Ireland, United Kingdom… (More)

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