Inactivation of wheat-germ aspartate transcarbamoylase by the triazinyl dye, procion red HE3B.

@article{Easton1992InactivationOW,
  title={Inactivation of wheat-germ aspartate transcarbamoylase by the triazinyl dye, procion red HE3B.},
  author={Michelle Easton and R. J. Yon},
  journal={Biochimica et biophysica acta},
  year={1992},
  volume={1118 3},
  pages={298-302}
}
Aspartate transcarbamoylase from wheat germ is irreversibly inactivated by the triazinyl dye Procion Red HE3B. Since triazinyl dyes may mimic nucleotides, and UMP is a known allosteric modifier of this enzyme, the reaction was studied to elucidate whether the dye is an 'affinity label' for the enzyme. The reaction is apparently first order in the first 5-10… CONTINUE READING