Inactivation of natural killer cell cytotoxic activity after interaction with target cells.

Human peripheral blood lymphocytes were cultured with unlabeled target cells or absorbed to K562 cells and then isolated by centrifugation on discontinuous Ficoll-Methrizoate gradient. In both cases, lymphocytes that reacted with these target cells at 37 degrees C were inactivated and were no longer able to kill freshly added 51Cr-labeled target cells that… CONTINUE READING