Inactivation of human normal and tumour cells irradiated with low energy protons.

@article{Belli2000InactivationOH,
  title={Inactivation of human normal and tumour cells irradiated with low energy protons.},
  author={M. Belli and D. Bettega and P. Calzolari and F. Cera and R. Cherubini and M. Dalla Vecchia and M. Durante and S. Favaretto and G. Gialanella and G. Grossi and R. Marchesini and G. Moschini and A. Piazzola and G. Poli and M. Pugliese and O. Sapora and P. Scampoli and G. Simone and E. Sorrentino and M. Tabocchini and L. Tallone and P. Tiveron},
  journal={International journal of radiation biology},
  year={2000},
  volume={76 6},
  pages={
          831-9
        }
}
PURPOSE To analyse the cell inactivation frequencies induced by low energy protons in human cells with different sensitivity to photon radiation. MATERIALS AND METHODS Four human cell lines with various sensitivities to photon irradiation were used: the SCC25 and SQ20B derived from human epithelium tumours of the tongue and larynx, respectively, and the normal lines M/10, derived from human mammary epithelium, and HF19 derived from a lung fibroblast. The cells were irradiated with y-rays and… Expand
Effects of low energy protons on clonogenic survival, DSB repair and cell cycle in human glioblastoma cells and B14 fibroblasts.
  • H. Moertel, J. Georgi, +4 authors R. Sauer
  • Chemistry, Medicine
  • Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology
  • 2004
TLDR
The effect of higher LET irradiation was more distinct for tumor cells than for normal tissue and both cell lines showed a higher RBE for cell survival than the value of 1.1 normally used for proton therapy. Expand
Inactivation of human cells exposed to fractionated doses of low energy protons: relationship between cell sensitivity and recovery efficiency.
Within the framework of radiation biophysics research in the hadrontherapy field, split-dose studies have been performed on four human cell lines with different radiation sensitivity (SCC25, HF19,Expand
Response of a Human Melanoma Cell Line to Low and High Ionizing Radiation
TLDR
Both irradiation qualities caused modest G2‐M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells. Expand
Early effects of gamma rays and protons on human melanoma cell viability and morphology
TLDR
Higher ionization level of protons with respect to gamma rays, representing the main physical difference between these two types of radiation, was also revealed on the cell membrane level through larger pro‐apoptotic capacity of protONS. Expand
Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam
TLDR
It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while γ-rays eliminate viable cells primarily by the production of irreparable DNA damage. Expand
Cell cycle perturbations and genotoxic effects in human primary fibroblasts induced by low-energy protons and X/gamma-rays.
TLDR
In normal human primary fibroblasts cell cycle arrest at the G(1)/S transition can be triggered shortly after irradiation and maintained for several hours post-irradiation of both protons and X-rays. Expand
Effectiveness of monoenergetic and spread-out bragg peak carbon-ions for inactivation of various normal and tumour human cell lines.
TLDR
The data show the superior effectiveness for cell-killing exhibited by carbon-ion beams compared to lower LET radiation, particularly in tumour cells radioresistant to X- or gamma-rays, hence the advantage of using such beams in radiotherapy. Expand
mFISH analysis of irradiated human fibroblasts: a comparison among radiations with different quality in the low-dose range.
The present investigation aimed to characterise the shape of dose-response curve and determining the frequency distribution of various aberration types as a function of dose and radiation quality inExpand
Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak
TLDR
Survival data confirmed very high radioresistance of HTB140 cells and an effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses, illustrated by rather high RBE. Expand
Investigating Dependencies of Relative Biological Effectiveness for Proton Therapy in Cancer Cells
TLDR
Proton RBE was found to depend on mean proton energy, survival end point, and cell type, and changes in both y‒D and LETd were also found to impact proton R BE values, but consideration of the energy spectrum may provide additional information. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 38 REFERENCES
Effects of α-particles on survial and chromosomal aberrations in human mammary epithelial cells
TLDR
The results suggest that α-particle-induced reproductive death of human mammary epithelial cells is caused by chromosome damage in the first 5 generations following exposure, whereas the in-activation produced by low-LET radiation is mostly related to the aberrations at the first post-irradiation mitosis. Expand
Biological effectiveness of low energy protons. I. Survival of Chinese hamster cells.
TLDR
It was concluded that in this low energy range the biological effectiveness increases substantially with decreasing proton energy. Expand
The irradiation of V79 mammalian cells by protons with energies below 2 MeV. Part I: Experimental arrangement and measurements of cell survival.
TLDR
Measurements of the survival of V79 Chinese hamster cells suggest that protons are most effective at about 40-50 keV microns-1, and it is shown that the proton RBEs can be reconciled with those of other light ions if plotted against z*2/beta 2 rather than against LET. Expand
Response of sensitive human ataxia and resistant T-1 cell lines to accelerated heavy ions.
TLDR
The repair-misrepair model suggests that at high LET the T-1 cells can still efficiently repair individual lesions, but that as the lesions become closely spaced along the tracks, the probability of misrepair increases. Expand
Irregular variations in radiation sensitivity when the linear energy transfer is increased.
TLDR
Seven cell lines were analyzed for clonogenic survival after irradiation with photons or accelerated helium or nitrogen ions and there was only a weak tendency towards a relation between photon and helium ion sensitivity when the linear component of the survival curves were compared. Expand
RBE-LET relationship for the survival of V79 cells irradiated with low energy protons.
TLDR
The data seem to indicate that the RBE-LET curve depends on the type of radiation and this could imply that LET is not a good reference for the dose-effectiveness relationship. Expand
The radiosensitivity of the chromosomes of the cells of human squamous cell carcinoma cell lines.
TLDR
There was no relationship between DNA content and radiation sensitivity as measured by the cell survival assay or the induction of chromosome aberrations, although cells with larger DNA contents tended to have more chromosome damage per cell at equitoxic doses. Expand
Mutation and inactivation of cultured mammalian cells exposed to beams of accelerated heavy ions. III. Human diploid fibroblasts.
  • R. Cox, W. Masson
  • Biology, Medicine
  • International journal of radiation biology and related studies in physics, chemistry, and medicine
  • 1979
TLDR
The induction of inactivation and mutation to thioguanine-resistance in cultured human diploid fibroblasts was studied after exposure to ionising radiations with LET's in the range 20--470 keV micrometer-1 to discuss possible selective effects against mutants during post-irradiation growth. Expand
Dose-rate effects on the survival of irradiated hypersensitive and normal human fibroblasts.
TLDR
Surprisingly, the alpha component decreased with decreasing dose-rate for all 5 cell lines (only in plateau phase), thus low dose-rates do not allow direct measurement of the alpha components; the decrease in alpha could be interpreted as adaptive radioresistance. Expand
Radiation-induced DNA double-strand break frequencies in human squamous cell carcinoma cell lines of different radiation sensitivities.
TLDR
Although the low-dose (5-25 Gy) elution results were variable, they do suggest that DNA neutral elution will detect differences between sensitive and resistant tumour cells in initial DNA dsb frequencies. Expand
...
1
2
3
4
...