Inactivation of human and simian rotaviruses by ozone

  title={Inactivation of human and simian rotaviruses by ozone},
  author={J. Vaughn and Y. S. Chen and K. Lindburg and D. Morales},
  journal={Applied and Environmental Microbiology},
  pages={2218 - 2221}
The inactivation of simian rotavirus SA-11 and human rotavirus type 2 (Wa) by ozone was compared at 4 degrees C by using single-particle virus stocks. Although the human strain was clearly more sensitive, both virus types were rapidly inactivated by ozone concentrations of 0.25 mg/liter or greater at all pH levels tested. Comparison of the virucidal activity of ozone with that of chlorine in identical experiments indicated little significant difference in rotavirus-inactivating efficiencies… Expand
Inactivation of human and simian rotaviruses by chlorine dioxide
The observed enhancement of virucidal efficiency with increasing pH was contrary to earlier findings with chlorine- and ozone-treated rotavirus particles, where efficiencies decreased with increasing alkalinity. Expand
Effects of ozone treatment on the infectivity of hepatitis A virus.
The inactivation of a large-focus-forming variant of hepatitis A virus (HM-175) by ozone was investigated and exposure of virus particles to ozone concentrations of 1 mg/L or greater at all pH levels resulted in their complete inactivation within 60 s. Expand
Susceptibility of human rotavirus to ozone, high pressure, and pulsed electric field.
The rotavirus was found to be resistant to PEF treatment at 20 to 29 kV/cm, for which no appreciable reductions in virus titer were observed. Expand
Disinfection of human enteric viruses in water by copper and silver in combination with low levels of chlorine
PV does not appear to be an adequate model viral strain to be used in disinfection studies, and Bacteroides fragilis bacteriophages were consistently more resistant to disinfection than PV, suggesting they would be more suitable indicators, although they survived significantly less than HAV or HRV. Expand
Effects of chlorine and chlorine dioxide on human rotavirus infectivity and genome stability.
The results collectively indicate that the current practice of chlorine disinfection may be inadequate to manage the risk of waterborne HRV infection, and offer the potential to monitor the infectivity of HRV adapting PCR-based protocols in chlorine disinfections. Expand
Proteolytic inactivation of simian-11 rotavirus: a pilot study.
Results show that the alkaline protease alcalase is capable of inactivating SA-11 virus to a certain degree depending on conditions, and this enzyme was the most effective of the various proteases tested. Expand
Reduction of Norwalk Virus, Poliovirus 1, and Bacteriophage MS2 by Ozone Disinfection of Water
It is indicated that Norwalk virus as well as other enteric viruses can be reduced rapidly and extensively by ozone disinfection and that RT-PCR is a useful surrogate assay for both culturable and nonculturable viruses disinfected with ozone. Expand
Inactivation of internalized and surface contaminated enteric viruses in green onions.
The results suggest that ozone and high pressure processes aimed to reduce the level of microbial contamination of produce have the ability to inactivate viruses if they become localized in the interior portions of produce. Expand
Infectivity and genome persistence of rotavirus and astrovirus in groundwater and surface water.
The enterobacterial content of the water as well as extrinsic factors, such as temperature and light, correlated with the stability of virus infectivity, and with the persistence of the virus genetic material, suggesting that molecular techniques to detect and quantify viral genomes would be suitable for the detection of viruses in water. Expand
Evaluation of the infectivity, gene and antigenicity persistence of rotaviruses by free chlorine disinfection.
It is suggested that re-evaluation of resistance of other waterborne viruses after disinfections by more sensitive infectivity detection method (such as ICC-RT-qPCR) may be necessary, to determine the adequate disinfectant doses required for the inactivation ofWaterborne viruses. Expand


Inactivation of human and simian rotaviruses by chlorine
The inactivation of simian rotav virus SA-11 and human rotavirus type 2 (Wa) by chlorine was compared at 4 degrees C by using single-particle virus stocks to study the chlorine sensitivities of both virus types. Expand
Mechanism of enteroviral inactivation by ozone
The mechanism of enteroviral inactivation by ozone was investigated with poliovirus 1 (Mahoney) as the model virus and it was concluded that the damage to the viral nucleic acid is the major cause of poliov virus 1 in activation by ozone. Expand
Comparison of Chlorine and Ozone as Virucidal Agents of Poliomyelitis Virus.∗
Conclusions and Summary 1. Gaseous chlorine and hypochlorite in residual amounts of 0.5 ppm failed to inactivate a 1:1000 dilution of Le strain of poliomyelitis virus after an exposure of 90 minutesExpand
Inactivation of poliovirus I (Brunhilde) single particles by chlorine in water
The Brunhilde strain of poliovirus aggregated slowly in dilute phosphate-carbonate buffer at pH 6 but not at all at or above pH 7, making the OCl- at pH 10 over three times more effective than HOCl at pH6. Expand
Measurement of the inactivation kinetics of poliovirus by ozone in a fast-flow mixer
An obvious dose-response relationship was demonstrated during the first step of the inactivation curve, and the pH of the water slightly affected the viral inactivation rate, but these small differences seem to have no practical value. Expand
Comparative response of mixed cultures of bacteria and virus to ozonation
Abstract The ozone disinfection studies were performed using five cultures of organisms: i.e. a yeast Candida parapsilosis . an acid-fast organism Mycobacterium fortuitum, Salmonella typhimurium,Expand
Inactivation of viruses and bacteria by ozone, with and without sonication.
Simultaneous treatments by ozonation and sonication reduced the contact time for complete inactivation of microorganisms in secondary effluent and resulted in a synergistic effect. Expand
Spectrophotometric method for determination of ozone in aqueous solutions
Abstract A sensitive spectrophotometric method for determination of ozone in small volumes of water is presented. It was performed in order to meet the requirements of a kinetic study of virus andExpand
Current state-of-the-art of wastewater disinfection
  • J. Water Pollut. Control Fed
  • 1983
  • Appi. Environ. Microbiol
  • 1979