Inactivation of cytochromes P450 2B protects against cocaine-mediated toxicity in rat liver slices.

@article{Poet1994InactivationOC,
  title={Inactivation of cytochromes P450 2B protects against cocaine-mediated toxicity in rat liver slices.},
  author={Torka S. Poet and Klaus Brendel and James R. Halpert},
  journal={Toxicology and applied pharmacology},
  year={1994},
  volume={126 1},
  pages={
          26-32
        }
}
Mechanism-based inactivators of rat liver cytochrome P450 2B1 and 2B2 were used to evaluate the role of these enzymes in the hepatotoxicity of cocaine. Loss of liver microsomal androstenedione 16 beta-hydroxylation was monitored to determine the extent of P450 2B1/2 inactivation by chloramphenicol (CAP) or its 2B-selective analogue, N-(2-p-nitrophenethyl)chlorofluoroacetamide (pNO2C1FA). The effect of P450 2B1/2 inactivation on cocaine-mediated hepatotoxicity was assessed in rat liver slices… CONTINUE READING