Inactivation of creatine kinase by high pressure may precede dimer dissociation.

@article{Zhou2000InactivationOC,
  title={Inactivation of creatine kinase by high pressure may precede dimer dissociation.},
  author={J. M. Zhou and Li-Min Zhu and Claude Balny},
  journal={European journal of biochemistry},
  year={2000},
  volume={267 4},
  pages={1247-53}
}
The effects of hydrostatic pressure on creatine kinase activity and conformation were investigated using either the high-pressure stopped-flow method in the pressure range 0.1-200 MPa for the activity determination, or the conventional activity measurement and fluorescence spectroscopy up to 650 MPa. The changes in creatine kinase activity and intrinsic fluorescence show a total or partial reversibility after releasing pressure, depending on both the initial value of the high pressure applied… CONTINUE READING