In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes.

@article{Rasmussen1993InVT,
  title={In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes.},
  author={Eric B Rasmussen and John T Lis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 17},
  pages={7923-7}
}
The regulation of many eukaryotic genes occurs at the level of transcriptional elongation. On the uninduced hsp70 gene of Drosophila melanogaster, for example, an RNA polymerase II complex has initiated transcription but has paused early in elongation. In this study, we examine pausing on hsp70 and two of the small heat shock genes (hsp27 and hsp26) at high resolution, using a technique that utilizes paramagnetic particle-mediated selection of terminated run-on transcripts. This technique… CONTINUE READING