In vivo transcription of a human antithrombin III "minigene".

  title={In vivo transcription of a human antithrombin III "minigene".},
  author={Edward V. Prochownik and Stuart H Orkin},
  journal={The Journal of biological chemistry},
  volume={259 24},
A 2.2-kilobase human antithrombin III (ATIII) "minigene" has been constructed which preserves many of the features of the 16-kilobase parental gene. These include approximately 300 base pairs of the 5'-flanking region containing the "TATA"-less ATIII promoter, the entire protein-coding sequence including the signal peptide, a single intervening sequence with intact donor and acceptor splice sites, and a polyadenylation signal. Unique PstI termini allow the minigene to be cloned in many commonly… CONTINUE READING

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