In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris.

@article{Vervecken2004InVS,
  title={In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris.},
  author={Wouter Vervecken and Vladimir Kaigorodov and Nico 3 Callewaert and Steven Geysens and Kristof De Vusser and Roland Contreras},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 5},
  pages={2639-46}
}
The Pichia pastoris N-glycosylation pathway is only partially homologous to the pathway in human cells. In the Golgi apparatus, human cells synthesize complex oligosaccharides, whereas Pichia cells form mannose structures that can contain up to 40 mannose residues. This hypermannosylation of secreted glycoproteins hampers the downstream processing of heterologously expressed glycoproteins and leads to the production of protein-based therapeutic agents that are rapidly cleared from the blood… CONTINUE READING

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