In vivo stoichiometry of shelterin components.

@article{Takai2010InVS,
  title={In vivo stoichiometry of shelterin components.},
  author={Kaori Kajihara Takai and Sarah Hooper and Stephanie Blackwood and Rita Gandhi and Titia de Lange},
  journal={The Journal of biological chemistry},
  year={2010},
  volume={285 2},
  pages={1457-67}
}
Human telomeres bind shelterin, the six-subunit protein complex that protects chromosome ends from the DNA damage response and regulates telomere length maintenance by telomerase. We used quantitative immunoblotting to determine the abundance and stoichiometry of the shelterin proteins in the chromatin-bound protein fraction of human cells. The abundance of shelterin components was similar in primary and transformed cells and was not correlated with telomere length. The duplex telomeric DNA… CONTINUE READING