In vivo silencing of a molecular target by short interfering RNA electroporation: tumor vascularization correlates to delivery efficiency.

@article{Takei2008InVS,
  title={In vivo silencing of a molecular target by short interfering RNA electroporation: tumor vascularization correlates to delivery efficiency.},
  author={Yoshifumi Takei and Toshio Nemoto and Ping Mu and Tatsuya Fujishima and Takuji Ishimoto and Yasuhiko Hayakawa and Yukio Yuzawa and Seiichi Matsuo and Takashi Muramatsu and Kenji Kadomatsu},
  journal={Molecular cancer therapeutics},
  year={2008},
  volume={7 1},
  pages={211-21}
}
Screening for a molecular target for cancer therapy requires multiple steps, of which an important one is evaluation of the knockdown effect of the target molecule on pregrown xenograft tumors. However, methods currently used for local administration of knockdown reagents, such as short interfering RNA (siRNA), are not satisfactory as to simplicity and efficiency. We established an electroporation method involving a constant voltage and "plate and fork" type electrodes and used it for in vivo… CONTINUE READING