In vivo random mutagenesis of Bacillus subtilis by use of TnYLB-1, a mariner-based transposon.

Abstract

This report describes the construction and characterization of a mariner-based transposon system designed to be used in Bacillus subtilis, but potentially applicable to other gram-positive bacteria. Two pUC19-derived plasmids were created that contain the mariner-Himar1 transposase gene, modified for expression in B. subtilis, under the control of either… (More)

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