In vivo pools of free and acylated acyl carrier proteins in spinach. Evidence for sites of regulation of fatty acid biosynthesis.

@article{PostBeittenmiller1991InVP,
  title={In vivo pools of free and acylated acyl carrier proteins in spinach. Evidence for sites of regulation of fatty acid biosynthesis.},
  author={Dusty Post-Beittenmiller and Jan G. Jaworski and John B. Ohlrogge},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 3},
  pages={1858-65}
}
In order to examine potential regulatory steps in plant fatty acid biosynthesis, we have developed procedures for the analysis of the major acyl-acyl carrier protein (ACP) intermediates of this pathway. These techniques have been used to separate and identify acyl-ACPs with chain configurations ranging from 2:0 to 18:1 and to determine the relative in vivo concentrations of acyl-ACPs in spinach leaf and developing seed. In both leaf and seed as much as 60% of the total ACPs were nonesterified… CONTINUE READING