In vivo optical imaging of early-stage apoptosis in mouse brain after transient cerebral ischemia.

@article{Liu2010InVO,
  title={In vivo optical imaging of early-stage apoptosis in mouse brain after transient cerebral ischemia.},
  author={Ning Liu and Kentaro Deguchi and Jingwei Shang and Xuemei Zhang and FengFeng Tian and Toru Yamashita and Yasuyuki Ohta and Yoshio T. Ikeda and Tohru Matsuura and Koji Abe},
  journal={Journal of neuroscience research},
  year={2010},
  volume={88 16},
  pages={
          3488-97
        }
}
Apoptosis is one of the mechanisms contributing to neuronal degeneration in ischemic stroke. In vivo imaging of annexin V (A5) was performed at 12 hr, 24 hr, 48 hr, and 4 days after 90-min transient middle cerebral artery occlusion (tMCAO) in mice with a fluorescent protein Cy5.5. Immunohistochemistry for heat shock protein 70 (HSP70), A5, and TUNEL were also performed with brain sections after the tMCAO. In vivo fluorescence was strongly observed at 48 hr over the head, especially with removal… CONTINUE READING