In vivo nuclear transport kinetics in Saccharomyces cerevisiae.

  title={In vivo nuclear transport kinetics in Saccharomyces cerevisiae.},
  author={Paul M. Roberts and David S. Goldfarb},
  journal={Methods in cell biology},
We have described a direct fluorescence assay to measure the relative rates of NLS-directed import and passive export of an NLS-GFP fusion protein in yeast. The design and construction of the reporter GFP fusion, its spectral qualities, size, use of inducible promoters, and the choice of NLS, are variables that could extend the method's utility. Future applications will almost certainly demand the quantification of transport rates in single cells using image analysis techniques. As is the case… CONTINUE READING

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