In vivo nitric oxide detection in the septic rat brain by electron paramagnetic resonance.

@article{Suzuki1998InVN,
  title={In vivo nitric oxide detection in the septic rat brain by electron paramagnetic resonance.},
  author={Yasuhiro Suzuki and Satoshi Fujii and Yoshihiro Numagami and Teiji Tominaga and Tanihiro Yoshimoto and Tetsuhiko Yoshimura},
  journal={Free radical research},
  year={1998},
  volume={28 3},
  pages={293-9}
}
To detect nitric oxide (NO) in the rat brain during lipopolysaccharide (LPS)-induced sepsis, electron paramagnetic resonance (EPR) was employed with the NO trapping technique, using an iron and N,N-diethyldithiocarbamate (DETC) complex. An X-band (about 9.5 GHz) EPR system detected a triplet signal (g = 2.038) derived from an NO-Fe-DETC complex being superimposed on the g(perpendicular) signal of Cu-DETC complex at liquid nitrogen temperature. The height of the triplet signal peaked seven hours… CONTINUE READING

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