In vivo modification of adriamycin-induced apoptosis in L-5178Y lymphoma cell-bearing mice by (+)-alpha-tocopherol and superoxide dismutase.

Abstract

Apoptosis was followed in L5178Y lymphoma cell-bearing mice at different times after intraperitoneal injections of adriamycin (ADM). Apoptosis was determined morphologically and confirmed by DNA laddering on electrophoresis. Apoptosis was observed 36h after injection of 5mg/kg ADM (apoptotic cell index 64.2+/-5.6 vs. 1.5+/-2.1 from the untreated group) and confirmed by DNA electrophoresis. However, when the animals were pretreated with (+)-alpha-tocopherol acid succinate or superoxide dismutase before ADM administration apoptotic index significantly diminished (P<0.05) and the DNA electrophoresis did not show fragmentations. We conclude that in ADM-treated mice, tumour cell death occurs in two ways: first by necrosis, then later by apoptosis. These observations are likely to be associated with or caused by the generation of reactive oxygen species.

Cite this paper

@article{BravoCullar2005InVM, title={In vivo modification of adriamycin-induced apoptosis in L-5178Y lymphoma cell-bearing mice by (+)-alpha-tocopherol and superoxide dismutase.}, author={Alejandro Bravo-Cu{\'e}llar and Piedad C G{\'o}mez-Contreras and Jos{\'e} Manuel Lerma-D{\'i}az and Georgina Hern{\'a}ndez-Flores and Jorge Ramiro Dom{\'i}nguez-Rodr{\'i}guez and Pablo C{\'e}sar Ort{\'i}z-Lazareno and Ram{\'o}n Cervantes-Mungu{\'i}a and Simone Orbach-Arbouys}, journal={Cancer letters}, year={2005}, volume={229 1}, pages={59-65} }