In vivo metabolic tracking of 14C-radiolabelled isoflavones in kudzu (Pueraria lobata) and red clover (Trifolium pratense) extracts

  title={In vivo metabolic tracking of 14C-radiolabelled isoflavones in kudzu (Pueraria lobata) and red clover (Trifolium pratense) extracts},
  author={Jonathan G. Mun and Michael D Grannan and Pamela J. Lachcik and Adam Reppert and Gad G. Yousef and Randy B. Rogers and Elsa M. Janle and Connie M. Weaver and Mary Ann Lila},
  journal={British Journal of Nutrition},
  pages={1523 - 1530}
Absorption, distribution and elimination of 14C-labelled isoflavone-containing extracts from kudzu (Pueraria lobata) root culture and red clover (Trifolium pratense) cell culture were investigated in an in vivo rat model. The predominant isoflavones in the kudzu extract were the glycosides puerarin, daidzin and malonyl daidzin, while in the red clover extract, the major isoflavones were formononetin and its derivatives, genistein and biochanin A, with radioactivities of 3·770 and 7·256 MBq/g… 

Tracking deposition of a 14C-radiolabeled kudzu hairy root-derived isoflavone-rich fraction into bone

It is demonstrated that kudzu isoflavones and metabolites are capable of reaching bone tissues, where they may contribute to the prevention of osteoporosis and the promotion of bone health.

The metabolism and analysis of isoflavones and other dietary polyphenols in foods and biological systems.

Critical questions about the absorption and tissue uptake of complex polyphenols such as the proanthocyanins can be answered by labeling thesepolyphenols with (14)C-sucrose in plant cell culture and then purifying them for use in animal experiments, routinely carried out with great sensitivity, specificity and quantification by LC-tandem mass spectrometry.

The intestinal absorption properties of flavonoids in Hippophaë rhamnoides extracts by an in situ single-pass intestinal perfusion model

The results indicated that IS, QU, and KA in TFH were absorbed site-dependently, and both enterohepatic circulation and intestinal flora could participate in their absorption processes.

Neutral Loss Ion Mapping Experiment Combined with Precursor Mass List and Dynamic Exclusion for Screening Unstable Malonyl Glucoside Conjugates

The study provided a method for analyzing the malonyl conjugates from complex matrices but also explored a way to trace other low amount components in TCMs.

Puerarin stimulates osteoblasts differentiation and bone formation through estrogen receptor, p38 MAPK, and Wnt/β-catenin pathways

Taken together, ER, p38 MAPK, and Wnt/β-catenin pathways were involved in puerarin-stimulated osteoblasts differentiation and bone formation.

Unraveling Anthocyanin Bioavailability for Human Health.

It is argued that the persistence of anthocyanin metabolites suggests enterohepatic recycling, leading to prolonged residence time, and supports the notion that Anthocyanins are far more bioavailable than previously suggested.

A mechanistic review of chinese medicine polyphenols on bone formation and resorption

The purpose of this review is to provide comprehensive information on the mechanisms underlying the relationship between traditional Chinese medicine polyphenols and bone formation or bone resorption.



Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures.

The isolated isoflavones isolated from kudzu root cultures were sufficiently (14)C-labeled to permit utilization for subsequent in vivo metabolic tracking studies.

In vitro production of radiolabeled red clover (Trifolium pratense) isoflavones

Varying levels and timing of copper chloride elicitor did not significantly affect isoflavone accumulation, and approximately 38% of the 14C-sucrose dose accumulated in the cells.

Identification of isoflavone glycosides in Pueraria lobata cultures by tandem mass spectrometry.

The molecular weights, interpretation of characteristic fragment ions obtained from HPLC-MS/MS and comparison with reported data allowed the putative identification of the isoflavone metabolites.

Identification of puerarin and its metabolites in rats by liquid chromatography-tandem mass spectrometry.

A sensitive and specific LC-ESI-MS/MS method for the determination of puerarin and its metabolites daidzein, dihydrodaidzesin, and equol was developed and suggests that this glucoside may enter tissues by specific transport pathways.

Tissue distribution of puerarin and its conjugated metabolites in rats assessed by liquid chromatography-tandem mass spectrometry.

Intestinal uptake and biliary excretion of the isoflavone genistein in rats.

The intestinal absorption, biliary excretion and metabolism of genistein, a potent and specific protein tyrosine kinase inhibitor that occurs naturally in soy foods, was examined in anesthetized, adult female rats fitted with indwelling biliary cannulas to indicate thatgenistein is highly bioavailable in rats and because of its enterohepatic circulation may accumulate within the gastrointestinal tract.

The effect of isoflavones extracted from red clover (Rimostil) on lipid and bone metabolism

  • Phillip B. Clifton-BlighRodney J. BaberGreg R. FulcherMaria-Liza NeryTerry Moreton
  • Medicine
  • 2001
The results show that the administration of an isoflavone combination extracted from red clover was associated with a significant increase in high-density lipoprotein cholesterol, a significant fall in apolipoprotein B, and asignificant increase in the predominantly cortical bone of the proximal radius and ulna after 6 months of treatment.

A comparative survey of leguminous plants as sources of the isoflavones, genistein and daidzein: implications for human nutrition and health.

The results indicate that the legumes, lupine (Lupinus spp.), fava bean, (Vicia faba), soybeans (Glycine max), kudzu (Pueraria lobata), and psoralea (Psoralee corylifolia), are excellent food sources for both genistein and daidzein.

Toxicokinetics of the phytoestrogen daidzein in female DA/Han rats

The plasma concentration–time curves after oral administration of daidzein to female DA/Han rats revealed pronounced interindividual differences and multiple peaks, pointing to extensive enterohepatic circulation and/or protracted absorption from the gastrointestinal tract, and to an uptake and storage of isoflavones and metabolites in tissues.

Red clover Trifolium pratense L. phytoestrogens: UV-B radiation increases isoflavone yield, and postharvest drying methods change the glucoside conjugate profiles.

  • E. SwinnyK. Ryan
  • Chemistry, Medicine
    Journal of agricultural and food chemistry
  • 2005
Exposure to enhanced UV-B radiation resulted in an increase in total formononetin and biochanin A isoflavone levels, indicating that harvest during a period of high ambient UV- B radiation may be appropriate for maximum yield.