In vivo metabolic tracking of 14C-radiolabelled isoflavones in kudzu (Pueraria lobata) and red clover (Trifolium pratense) extracts

@article{Mun2009InVM,
  title={In vivo metabolic tracking of 14C-radiolabelled isoflavones in kudzu (Pueraria lobata) and red clover (Trifolium pratense) extracts},
  author={Jonathan G. Mun and Michael D Grannan and Pamela J. Lachcik and Adam Reppert and Gad G. Yousef and Randy B. Rogers and Elsa M. Janle and Connie M. Weaver and Mary Ann Lila},
  journal={British Journal of Nutrition},
  year={2009},
  volume={102},
  pages={1523 - 1530}
}
Absorption, distribution and elimination of 14C-labelled isoflavone-containing extracts from kudzu (Pueraria lobata) root culture and red clover (Trifolium pratense) cell culture were investigated in an in vivo rat model. The predominant isoflavones in the kudzu extract were the glycosides puerarin, daidzin and malonyl daidzin, while in the red clover extract, the major isoflavones were formononetin and its derivatives, genistein and biochanin A, with radioactivities of 3·770 and 7·256 MBq/g… Expand
9 Citations
Tracking deposition of a 14C-radiolabeled kudzu hairy root-derived isoflavone-rich fraction into bone
TLDR
It is demonstrated that kudzu isoflavones and metabolites are capable of reaching bone tissues, where they may contribute to the prevention of osteoporosis and the promotion of bone health. Expand
The metabolism and analysis of isoflavones and other dietary polyphenols in foods and biological systems.
TLDR
Critical questions about the absorption and tissue uptake of complex polyphenols such as the proanthocyanins can be answered by labeling thesepolyphenols with (14)C-sucrose in plant cell culture and then purifying them for use in animal experiments, routinely carried out with great sensitivity, specificity and quantification by LC-tandem mass spectrometry. Expand
The intestinal absorption properties of flavonoids in Hippophaë rhamnoides extracts by an in situ single-pass intestinal perfusion model
TLDR
The results indicated that IS, QU, and KA in TFH were absorbed site-dependently, and both enterohepatic circulation and intestinal flora could participate in their absorption processes. Expand
Neutral Loss Ion Mapping Experiment Combined with Precursor Mass List and Dynamic Exclusion for Screening Unstable Malonyl Glucoside Conjugates
  • Min Yang, Zhe Zhou, +7 authors D. Guo
  • Chemistry, Medicine
  • Journal of The American Society for Mass Spectrometry
  • 2015
TLDR
The study provided a method for analyzing the malonyl conjugates from complex matrices but also explored a way to trace other low amount components in TCMs. Expand
Puerarin stimulates osteoblasts differentiation and bone formation through estrogen receptor, p38 MAPK, and Wnt/β-catenin pathways
TLDR
Taken together, ER, p38 MAPK, and Wnt/β-catenin pathways were involved in puerarin-stimulated osteoblasts differentiation and bone formation. Expand
Drug enterohepatic circulation and disposition: constituents of systems pharmacokinetics.
TLDR
This work critically evaluate the existing methods, techniques and animal models used for determining drug distribution, elimination and EHC, and collectively portray characteristics of 43 drugs that undergo EHC. Expand
Unraveling Anthocyanin Bioavailability for Human Health.
TLDR
It is argued that the persistence of anthocyanin metabolites suggests enterohepatic recycling, leading to prolonged residence time, and supports the notion that Anthocyanins are far more bioavailable than previously suggested. Expand

References

SHOWING 1-10 OF 33 REFERENCES
Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures.
TLDR
The isolated isoflavones isolated from kudzu root cultures were sufficiently (14)C-labeled to permit utilization for subsequent in vivo metabolic tracking studies. Expand
In vitro production of radiolabeled red clover (Trifolium pratense) isoflavones
TLDR
Varying levels and timing of copper chloride elicitor did not significantly affect isoflavone accumulation, and approximately 38% of the 14C-sucrose dose accumulated in the cells. Expand
Identification of isoflavone glycosides in Pueraria lobata cultures by tandem mass spectrometry.
TLDR
The molecular weights, interpretation of characteristic fragment ions obtained from HPLC-MS/MS and comparison with reported data allowed the putative identification of the isoflavone metabolites. Expand
Identification of puerarin and its metabolites in rats by liquid chromatography-tandem mass spectrometry.
TLDR
A sensitive and specific LC-ESI-MS/MS method for the determination of puerarin and its metabolites daidzein, dihydrodaidzesin, and equol was developed and suggests that this glucoside may enter tissues by specific transport pathways. Expand
Tissue distribution of puerarin and its conjugated metabolites in rats assessed by liquid chromatography-tandem mass spectrometry.
TLDR
Identifying puerarin in several organs including kidney and pancreas may explain its beneficial effects in diabetes. Expand
Intestinal uptake and biliary excretion of the isoflavone genistein in rats.
TLDR
The intestinal absorption, biliary excretion and metabolism of genistein, a potent and specific protein tyrosine kinase inhibitor that occurs naturally in soy foods, was examined in anesthetized, adult female rats fitted with indwelling biliary cannulas to indicate thatgenistein is highly bioavailable in rats and because of its enterohepatic circulation may accumulate within the gastrointestinal tract. Expand
The effect of isoflavones extracted from red clover (Rimostil) on lipid and bone metabolism
TLDR
The results show that the administration of an isoflavone combination extracted from red clover was associated with a significant increase in high-density lipoprotein cholesterol, a significant fall in apolipoprotein B, and asignificant increase in the predominantly cortical bone of the proximal radius and ulna after 6 months of treatment. Expand
A comparative survey of leguminous plants as sources of the isoflavones, genistein and daidzein: implications for human nutrition and health.
TLDR
The results indicate that the legumes, lupine (Lupinus spp.), fava bean, (Vicia faba), soybeans (Glycine max), kudzu (Pueraria lobata), and psoralea (Psoralee corylifolia), are excellent food sources for both genistein and daidzein. Expand
Toxicokinetics of the phytoestrogen daidzein in female DA/Han rats
TLDR
The plasma concentration–time curves after oral administration of daidzein to female DA/Han rats revealed pronounced interindividual differences and multiple peaks, pointing to extensive enterohepatic circulation and/or protracted absorption from the gastrointestinal tract, and to an uptake and storage of isoflavones and metabolites in tissues. Expand
Red clover Trifolium pratense L. phytoestrogens: UV-B radiation increases isoflavone yield, and postharvest drying methods change the glucoside conjugate profiles.
TLDR
Exposure to enhanced UV-B radiation resulted in an increase in total formononetin and biochanin A isoflavone levels, indicating that harvest during a period of high ambient UV- B radiation may be appropriate for maximum yield. Expand
...
1
2
3
4
...