In vivo labeling of fission yeast DNA with thymidine and thymidine analogs.

@article{Sivakumar2004InVL,
  title={In vivo labeling of fission yeast DNA with thymidine and thymidine analogs.},
  author={Sasirekha Sivakumar and Mary E Porter-Goff and Prasanta K Patel and Kristen Benoit and Nicholas Rhind},
  journal={Methods},
  year={2004},
  volume={33 3},
  pages={213-9}
}
In vivo labeling of DNA with thymidine and thymidine analogs has long been a cornerstone of replication studies. Unfortunately, yeast lack a thymidine salvage pathway and thus do not incorporate exogenous thymidine. Specifically, yeast neither efficiently take up exogenous thymidine from their growth media nor phosphorylate it to thymidylate, the precursor of dTTP. We have overcome these problems in fission yeast by expressing the human equilibrative nucleoside transporter 1 (hENT1) along with… CONTINUE READING

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