In vivo labeling of B16 melanoma tumor xenograft with a thiol-reactive gadolinium based MRI contrast agent.

@article{Menchise2011InVL,
  title={In vivo labeling of B16 melanoma tumor xenograft with a thiol-reactive gadolinium based MRI contrast agent.},
  author={Valeria Menchise and Giuseppe Digilio and Eliana Gianolio and Evelina Cittadino and Valeria Catanzaro and Carla Carrera and Silvio Aime},
  journal={Molecular pharmaceutics},
  year={2011},
  volume={8 5},
  pages={1750-6}
}
Murine melanoma B16 cells display on the extracellular side of the plasma membrane a large number of reactive protein thiols (exofacial protein thiols, EPTs). These EPTs can be chemically labeled with Gd-DO3A-PDP, a Gd(III)-based MRI contrast agent bearing a 2-pyridinedithio chemical function for the recognition of EPTs. Uptake of gadolinium up to 10(9) Gd atoms per cell can be achieved. The treatment of B16 cells ex vivo with a reducing agent such as tris(2-carboxyethyl)phosphine (TCEP… CONTINUE READING

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These EPTs can be chemically labeled with Gd - DO3A - PDP , a Gd(III)-based MRI contrast agent bearing a 2-pyridinedithio chemical function for the recognition of EPTs .
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