In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography.

@article{Bassi2011InVL,
  title={In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography.},
  author={Andrea Bassi and Luca Fieramonti and Cosimo D'Andrea and Maria Caterina Mione and Gianluca Valentini},
  journal={Journal of biomedical optics},
  year={2011},
  volume={16 10},
  pages={100502}
}
We introduce flow optical projection tomography, an imaging technique capable of visualizing the vasculature of living specimens in 3-D. The method detects the movement of cells in the bloodstream and creates flow maps using a motion-analysis procedure. Then, flow maps obtained from projection taken at several angles are used to reconstruct sections of the circulatory system of the specimen. We therefore demonstrate an in vivo, 3-D optical imaging technique that, without the use of any labeling… CONTINUE READING