In vivo kinetics of mRNA splicing and transport in mammalian cells.

@article{Audibert2002InVK,
  title={In vivo kinetics of mRNA splicing and transport in mammalian cells.},
  author={Agn{\`e}s Audibert and D. Weil and F. Dautry},
  journal={Molecular and cellular biology},
  year={2002},
  volume={22 19},
  pages={6706-18}
}
The kinetics of pre-mRNA processing in living cells is poorly known, preventing a detailed analysis of the regulation of these reactions. Using tetracycline-regulated promoters we performed, during a transcriptional induction, a complete analysis of the maturation of two cellular mRNAs, those for LT-alpha and beta-globin. In both cases, splicing was appropriately described by first-order reactions with corresponding half-lives ranging between 0.4 and 7.5 min, depending on the intron. Transport… CONTINUE READING

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