In vivo investigation of the transcription, processing, endonucleolytic activity, and functional relevance of the spatial distribution of a plant miRNA.

@article{Parizotto2004InVI,
  title={In vivo investigation of the transcription, processing, endonucleolytic activity, and functional relevance of the spatial distribution of a plant miRNA.},
  author={Eneida Abreu Parizotto and Patrice Dunoyer and Nadia Rahm and Christophe Himber and Olivier Voinnet},
  journal={Genes & development},
  year={2004},
  volume={18 18},
  pages={2237-42}
}
We show, with miR171, that plant miRNA genes are modular independent transcription units in which the fold-back pre-miRNA is sufficient for miRNA processing, and that the upstream region contains highly specific promoter elements. Processing depends on flanking sequences within the miRNA stem-loop precursor rather than the miRNA sequence itself, and mutations affecting target pairing at the center and 5' but not 3' region of the miRNA compromise its function in vivo. Inactivation of the SDE1… CONTINUE READING
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