In vivo insertional mutagenesis in Corynebacterium pseudotuberculosis: an efficient means to identify DNA sequences encoding exported proteins.

@article{Dorella2006InVI,
  title={In vivo insertional mutagenesis in Corynebacterium pseudotuberculosis: an efficient means to identify DNA sequences encoding exported proteins.},
  author={Fernanda Alves Dorella and Estela M Estevam and Luis Gustavo Carvalho Pacheco and C. Guimaraes and Ubiraci G P Lana and Eliane Aparecida Gomes and Michele Mendes Barsante and Sergio Costa Oliveira and Roberto Meyer and Anderson Miyoshi and Vasco Azevedo},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 11},
  pages={7368-72}
}
The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport subunits and for hypothetical and unknown… CONTINUE READING