In vivo immobilization of enzymatically active polypeptides on the cell surface of Staphylococcus carnosus

@article{Strauss1996InVI,
  title={In vivo immobilization of enzymatically active polypeptides on the cell surface of Staphylococcus carnosus},
  author={A Strauss and Friedrich G{\"o}tz},
  journal={Molecular Microbiology},
  year={1996},
  volume={21}
}
Many surface proteins of Gram‐positive bacteria are covalently anchored to the cell wall by a ubiquitous mechanism, involving a specific, C‐terminal sorting signal. To achieve cell‐wall immobilization of a normally secreted enzyme in vivo, we constructed a hybrid protein consisting of Staphylococcus hyicus lipase and the C‐terminal region of Staphylococcus aureus fibronectin binding protein B (FnBPB). This region comprised the authentic cell‐wall‐spanning region and cell‐wall sorting signal of… 
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