In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation

@article{Scabini2010InVI,
  title={In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation},
  author={Matteo Scabini and Fabio Franco Stellari and Paolo Cappella and Sara Rizzitano and Gemma Texid{\'o} and Enrico Pesenti},
  journal={Apoptosis},
  year={2010},
  volume={16},
  pages={198-207}
}
In vivo imaging of apoptosis in a preclinical setting in anticancer drug development could provide remarkable advantages in terms of translational medicine. So far, several imaging technologies with different probes have been used to achieve this goal. Here we describe a bioluminescence imaging approach that uses a new formulation of Z-DEVD-aminoluciferin, a caspase 3/7 substrate, to monitor in vivo apoptosis in tumor cells engineered to express luciferase. Upon apoptosis induction, Z-DEVD… CONTINUE READING