In vivo human corneal confocal microscopy of identical fields of subepithelial nerve plexus, basal epithelial, and wing cells at different times.

A technique is described to obtain time-lapse reflected light confocal images of cells in the basal epithelium and adjacent wing cell layer from the in vivo human cornea. The technique is based on the sequential relocation of the unique patterns of the subepithelial nerve plexuses immediately posterior to Bowman's membrane. The patterns of individual… CONTINUE READING