In vivo half-life of a protein is a function of its amino-terminal residue.

@article{Bachmair1986InVH,
  title={In vivo half-life of a protein is a function of its amino-terminal residue.},
  author={A Bachmair and Daniel Daniel Finley and Alexander Varshavsky},
  journal={Science},
  year={1986},
  volume={234 4773},
  pages={179-86}
}
When a chimeric gene encoding a ubiquitin-beta-galactosidase fusion protein is expressed in the yeast Saccharomyces cerevisiae, ubiquitin is cleaved off the nascent fusion protein, yielding a deubiquitinated beta-galactosidase (beta gal). With one exception, this cleavage takes place regardless of the nature of the amino acid residue of beta gal at the ubiquitin-beta gal junction, thereby making it possible to expose different residues at the amino-termini of the otherwise identical beta gal… CONTINUE READING
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