In vivo genome editing using Staphylococcus aureus Cas9

@inproceedings{Ran2015InVG,
  title={In vivo genome editing using Staphylococcus aureus Cas9},
  author={Fei Ann Ran and L{\^e} Ch{\'i} C{\^o}ng and Winston X Yan and David Allen Scott and Jonathan S Gootenberg and Andrea J. Kriz and Bernd Zetsche and Ophir Shalem and Xuebing Wu and Kira S. Makarova and Eugene V. Koonin and Phillip A. Sharp and Feng Zhang},
  booktitle={Nature},
  year={2015}
}
The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform. However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) limits its utility for basic research and therapeutic applications that use the highly versatile adeno-associated virus (AAV) delivery vehicle. Here, we characterize six smaller Cas9 orthologues and show that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being more… CONTINUE READING
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