In vivo gene transfection via intravitreal injection of cationic liposome/plasmid DNA complexes in rabbits.

@article{Kawakami2004InVG,
  title={In vivo gene transfection via intravitreal injection of cationic liposome/plasmid DNA complexes in rabbits.},
  author={Shigeru Kawakami and Ayaka Harada and Koji Sakanaka and Koyo Nishida and Junzo Nakamura and Toshiyuki Sakaeda and Nobuhiro Ichikawa and Mikiro Nakashima and Hitoshi Sasaki},
  journal={International journal of pharmaceutics},
  year={2004},
  volume={278 2},
  pages={255-62}
}
To optimize the in vivo ocular transfection efficiency of plasmid DNA (pDNA)/cationic liposome complexes, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA)/dioleoylphosphatidylethanolamine (DOPE) (1:1 molar ratio) liposomes and DOTMA/cholesterol (Chol) (1:1 molar ratio) liposomes were prepared with varying amounts of pDNA. pDNA/cationic liposome complexes were intravitreally injected (100 microL) in rabbits, and luciferase activity in the cornea, aqueous humor, iris-ciliary… CONTINUE READING