In vivo functional analysis of in vitro protein binding sites in the immunoglobulin heavy chain enhancer.

@article{Tsao1988InVF,
  title={In vivo functional analysis of in vitro protein binding sites in the immunoglobulin heavy chain enhancer.},
  author={Betty P. Tsao and Xiaofei F Wang and Craig L. Peterson and Kathryn L. Calame},
  journal={Nucleic acids research},
  year={1988},
  volume={16 8},
  pages={3239-53}
}
We have systematically investigated the functional role of protein binding sites within the mouse immunoglobulin heavy chain enhancer which we previously identified by in vitro binding studies (1,2). Each binding site was deleted, mutant enhancers were cloned 3' of the chloramphenicol acetyl transferase gene in the vector pA10CAT2 and transfected into plasmacytoma cells. We demonstrate that the newly identified site E, located at 324-338 bp, is important for enhancer function; previously… CONTINUE READING

References

Publications referenced by this paper.

Genetic Engineering: Principles and Methods (Plenum

M A., W. Gilbert
New York), • 1980

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