In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase.

@article{Peters2006InVE,
  title={In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase.},
  author={Verena Peters and Bernd H A Rehm},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 3},
  pages={1777-83}
}
This study demonstrated that engineered polyhydroxyalkanoate (PHA) synthases can be employed as molecular tools to covalently immobilize enzymes at the PHA granule surface. The beta-galactosidase was fused to the N terminus of the class II PHA synthase from Pseudomonas aeruginosa. The open reading frame was confirmed to encode the complete fusion protein by T7 promoter-dependent overexpression. Restoration of PHA biosynthesis in the PHA-negative mutant of P. aeruginosa PAO1 showed a PHA… CONTINUE READING
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