In vivo enhancer analysis of human conserved non-coding sequences

@article{Pennacchio2006InVE,
  title={In vivo enhancer analysis of human conserved non-coding sequences},
  author={Len A. Pennacchio and Nadav Ahituv and Alan M. Moses and Shyam Prabhakar and Marcelo A. N{\'o}brega and Malak Shoukry and Simon Minovitsky and Inna Dubchak and Amy Holt and Keith D. Lewis and Ingrid Plajzer-Frick and Jennifer A. Akiyama and Sarah De Val and Veena Afzal and Brian L. Black and Olivier Couronne and Michael B. Eisen and Axel Visel and Edward M. Rubin},
  journal={Nature},
  year={2006},
  volume={444},
  pages={499-502}
}
Identifying the sequences that direct the spatial and temporal expression of genes and defining their function in vivo remains a significant challenge in the annotation of vertebrate genomes. One major obstacle is the lack of experimentally validated training sets. In this study, we made use of extreme evolutionary sequence conservation as a filter to identify putative gene regulatory elements, and characterized the in vivo enhancer activity of a large group of non-coding elements in the human… 
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