In vivo effect of DNA relaxation on the transcription of gene rpoH in Escherichia coli.

@article{LpezSnchez1997InVE,
  title={In vivo effect of DNA relaxation on the transcription of gene rpoH in Escherichia coli.},
  author={F L{\'o}pez-S{\'a}nchez and Jes{\'u}s Ram{\'i}rez-Santos and M. Carmen G{\'o}mez‐Eichelmann},
  journal={Biochimica et biophysica acta},
  year={1997},
  volume={1353 1},
  pages={
          79-83
        }
}
The in vivo effect of Novobiocin, a gyrase inhibitor, on the transcription of gene rpoH which codes for sigma32, the main positive regulator of the heat-shock response, was studied. Novobiocin induced a three-fold increase and a slight decrease in the activity of the rpoH promoters P1 and P4, respectively. The Novobiocin-induced increase in the activity of promoter P1 correlates with an increase in the amount of proteins sigma32 and DnaK. These results suggest that the increase in expression of… Expand
Analysis of the regulatory region of the heat-shock gene rpoH of Escherichia coli strains isolated from non-human hosts.
The regulatory region of the gene for sigma32, rpoH, of Escherichia coli strains isolated from non-human hosts and different geographic regions, was sequenced and compared with that of E. coli K12.Expand
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TLDR
The isolation of a novel sigma factor of about 24 kilodaltons that allows core RNA polymerase to transcribe preferentially from one of these promoters, rpoH3p, suggests that induction of heat shock proteins by the stringent response is not mediated by increased transcription of the rPOH gene. Expand
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TLDR
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