In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy.

Abstract

The authors demonstrate in vivo dynamic process imaging using a label-free real-time optical-resolution photoacoustic microscope (OR-PAM). This reflection-mode system takes advantage of a 532-nm fiber laser source with a high pulse repetition rate of up to 600 kHz combined with a fast-scanning mirror system. Microvasculature in SCID mouse ears is imaged at near real-time (0.5 fps) for a 1×1 mm2 field of view (FOV) with micron-scale lateral resolution. We also demonstrate imaging of cardiac-induced microhemodynamics in murine microvasculature at real-time frame-rates (30 fps) over a 250×250 μm2 FOV using real-time C-scan OR-PAM with ability to provide sustained imaging with near real-time feedback for focusing and positioning.

DOI: 10.1117/1.JBO.18.2.026001

3 Figures and Tables

Cite this paper

@article{Shi2013InVD, title={In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy.}, author={Wei Lin Shi and Peng Shao and Parsin Hajireza and Alexander E. Forbrich and Roger J. Zemp}, journal={Journal of biomedical optics}, year={2013}, volume={18 2}, pages={26001} }