In vivo duplication of genetic elements by the formation of stem-loop DNA without an RNA intermediate.

@article{Ohshima1992InVD,
  title={In vivo duplication of genetic elements by the formation of stem-loop DNA without an RNA intermediate.},
  author={A. Ohshima and Sumiko Inouye and M. Inouye},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 3},
  pages={1016-20}
}
Gene duplication through cDNA synthesis by reverse transcriptase is believed to have played an important role in the diversification of genomes during evolution. Here, we demonstrate that a genomic DNA sequence can be duplicated in vivo as a result of template switching. When an inverted repeat (IR) structure was inserted in a site downstream from a ColE1 plasmid origin of DNA replication, transformation of Escherichia coli cells with this plasmid resulted in the production of a new DNA… CONTINUE READING